normal human ascs Search Results


normal human asc  (ATCC)
96
ATCC normal human asc
Noggin protein increases alkaline phosphatase (ALP) activity and early osteogenic genes expression in adult human mesenchymal stem cells from different tissues. Alkaline phosphatase (ALP) activity after 7-d culture of ( a ) normal human bone marrow stromal cells (BMSCs), ( b ) normal human dental pulp stem cells (DPSCs) and ( c ) human immortalized adipose-derived stem cell <t>line</t> <t>(ASC52telo).</t> Cells were treated with either 100 ng/ml recombinant human Noggin (NOG) or 100 ng/ml recombinant human bone morphogenetic protein 2 (BMP-2), or both (BMP-2 + NOG), in osteogenic medium containing ascorbic acid <t>(Asc)</t> and dexamethasone (Dex). ( d ) ALP activity after 7-d culture of ASC52telo cells in osteogenic medium with different Noggin doses (100–400 ng/ml). ( a–d ) Control represents cells maintained in standard growth medium. Relative mRNA levels (qPCR) of selected osteoblastic markers in ( e ) normal human ASCs and ( f ) normal human BMSCs continuously treated with Noggin (NOG) or BMP-2 for 7 days in osteogenic medium. Relative quantification to control cells cultured in osteogenic medium. ( a–f ) Average values ± SD are plotted. One-way ANOVA tests, * p < 0.05, ** p < 0.001, *** p < 0.0001, ns—not significant, relative to respective control or between marked groups.
Normal Human Asc, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normal human asc - by Bioz Stars, 2026-04
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na v 1 1  (Alomone Labs)
95
Alomone Labs na v 1 1
Noggin protein increases alkaline phosphatase (ALP) activity and early osteogenic genes expression in adult human mesenchymal stem cells from different tissues. Alkaline phosphatase (ALP) activity after 7-d culture of ( a ) normal human bone marrow stromal cells (BMSCs), ( b ) normal human dental pulp stem cells (DPSCs) and ( c ) human immortalized adipose-derived stem cell <t>line</t> <t>(ASC52telo).</t> Cells were treated with either 100 ng/ml recombinant human Noggin (NOG) or 100 ng/ml recombinant human bone morphogenetic protein 2 (BMP-2), or both (BMP-2 + NOG), in osteogenic medium containing ascorbic acid <t>(Asc)</t> and dexamethasone (Dex). ( d ) ALP activity after 7-d culture of ASC52telo cells in osteogenic medium with different Noggin doses (100–400 ng/ml). ( a–d ) Control represents cells maintained in standard growth medium. Relative mRNA levels (qPCR) of selected osteoblastic markers in ( e ) normal human ASCs and ( f ) normal human BMSCs continuously treated with Noggin (NOG) or BMP-2 for 7 days in osteogenic medium. Relative quantification to control cells cultured in osteogenic medium. ( a–f ) Average values ± SD are plotted. One-way ANOVA tests, * p < 0.05, ** p < 0.001, *** p < 0.0001, ns—not significant, relative to respective control or between marked groups.
Na V 1 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/na v 1 1/product/Alomone Labs
Average 95 stars, based on 1 article reviews
na v 1 1 - by Bioz Stars, 2026-04
95/100 stars
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anp ms igg biogenesis  (Alomone Labs)
90
Alomone Labs anp ms igg biogenesis
Noggin protein increases alkaline phosphatase (ALP) activity and early osteogenic genes expression in adult human mesenchymal stem cells from different tissues. Alkaline phosphatase (ALP) activity after 7-d culture of ( a ) normal human bone marrow stromal cells (BMSCs), ( b ) normal human dental pulp stem cells (DPSCs) and ( c ) human immortalized adipose-derived stem cell <t>line</t> <t>(ASC52telo).</t> Cells were treated with either 100 ng/ml recombinant human Noggin (NOG) or 100 ng/ml recombinant human bone morphogenetic protein 2 (BMP-2), or both (BMP-2 + NOG), in osteogenic medium containing ascorbic acid <t>(Asc)</t> and dexamethasone (Dex). ( d ) ALP activity after 7-d culture of ASC52telo cells in osteogenic medium with different Noggin doses (100–400 ng/ml). ( a–d ) Control represents cells maintained in standard growth medium. Relative mRNA levels (qPCR) of selected osteoblastic markers in ( e ) normal human ASCs and ( f ) normal human BMSCs continuously treated with Noggin (NOG) or BMP-2 for 7 days in osteogenic medium. Relative quantification to control cells cultured in osteogenic medium. ( a–f ) Average values ± SD are plotted. One-way ANOVA tests, * p < 0.05, ** p < 0.001, *** p < 0.0001, ns—not significant, relative to respective control or between marked groups.
Anp Ms Igg Biogenesis, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
anp ms igg biogenesis - by Bioz Stars, 2026-04
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human ascs (hasc)  (Wolters Kluwer Health)
90
Wolters Kluwer Health human ascs (hasc)
Noggin protein increases alkaline phosphatase (ALP) activity and early osteogenic genes expression in adult human mesenchymal stem cells from different tissues. Alkaline phosphatase (ALP) activity after 7-d culture of ( a ) normal human bone marrow stromal cells (BMSCs), ( b ) normal human dental pulp stem cells (DPSCs) and ( c ) human immortalized adipose-derived stem cell <t>line</t> <t>(ASC52telo).</t> Cells were treated with either 100 ng/ml recombinant human Noggin (NOG) or 100 ng/ml recombinant human bone morphogenetic protein 2 (BMP-2), or both (BMP-2 + NOG), in osteogenic medium containing ascorbic acid <t>(Asc)</t> and dexamethasone (Dex). ( d ) ALP activity after 7-d culture of ASC52telo cells in osteogenic medium with different Noggin doses (100–400 ng/ml). ( a–d ) Control represents cells maintained in standard growth medium. Relative mRNA levels (qPCR) of selected osteoblastic markers in ( e ) normal human ASCs and ( f ) normal human BMSCs continuously treated with Noggin (NOG) or BMP-2 for 7 days in osteogenic medium. Relative quantification to control cells cultured in osteogenic medium. ( a–f ) Average values ± SD are plotted. One-way ANOVA tests, * p < 0.05, ** p < 0.001, *** p < 0.0001, ns—not significant, relative to respective control or between marked groups.
Human Ascs (Hasc), supplied by Wolters Kluwer Health, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
human ascs (hasc) - by Bioz Stars, 2026-04
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human colon ascending membrane tissue lysate (adult membrane normal)  (Novus Biologicals)
N/A
Human Colon ascending Membrane Tissue Lysate (Adult Membrane Normal)
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human colon ascending whole tissue lysate (adult whole normal)  (Novus Biologicals)
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Human Colon ascending Whole Tissue Lysate (Adult Whole Normal)
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Image Search Results


Noggin protein increases alkaline phosphatase (ALP) activity and early osteogenic genes expression in adult human mesenchymal stem cells from different tissues. Alkaline phosphatase (ALP) activity after 7-d culture of ( a ) normal human bone marrow stromal cells (BMSCs), ( b ) normal human dental pulp stem cells (DPSCs) and ( c ) human immortalized adipose-derived stem cell line (ASC52telo). Cells were treated with either 100 ng/ml recombinant human Noggin (NOG) or 100 ng/ml recombinant human bone morphogenetic protein 2 (BMP-2), or both (BMP-2 + NOG), in osteogenic medium containing ascorbic acid (Asc) and dexamethasone (Dex). ( d ) ALP activity after 7-d culture of ASC52telo cells in osteogenic medium with different Noggin doses (100–400 ng/ml). ( a–d ) Control represents cells maintained in standard growth medium. Relative mRNA levels (qPCR) of selected osteoblastic markers in ( e ) normal human ASCs and ( f ) normal human BMSCs continuously treated with Noggin (NOG) or BMP-2 for 7 days in osteogenic medium. Relative quantification to control cells cultured in osteogenic medium. ( a–f ) Average values ± SD are plotted. One-way ANOVA tests, * p < 0.05, ** p < 0.001, *** p < 0.0001, ns—not significant, relative to respective control or between marked groups.

Journal: Scientific Reports

Article Title: Noggin promotes osteogenesis in human adipose-derived mesenchymal stem cells via FGFR2/Src/Akt and ERK signaling pathway

doi: 10.1038/s41598-024-56858-w

Figure Lengend Snippet: Noggin protein increases alkaline phosphatase (ALP) activity and early osteogenic genes expression in adult human mesenchymal stem cells from different tissues. Alkaline phosphatase (ALP) activity after 7-d culture of ( a ) normal human bone marrow stromal cells (BMSCs), ( b ) normal human dental pulp stem cells (DPSCs) and ( c ) human immortalized adipose-derived stem cell line (ASC52telo). Cells were treated with either 100 ng/ml recombinant human Noggin (NOG) or 100 ng/ml recombinant human bone morphogenetic protein 2 (BMP-2), or both (BMP-2 + NOG), in osteogenic medium containing ascorbic acid (Asc) and dexamethasone (Dex). ( d ) ALP activity after 7-d culture of ASC52telo cells in osteogenic medium with different Noggin doses (100–400 ng/ml). ( a–d ) Control represents cells maintained in standard growth medium. Relative mRNA levels (qPCR) of selected osteoblastic markers in ( e ) normal human ASCs and ( f ) normal human BMSCs continuously treated with Noggin (NOG) or BMP-2 for 7 days in osteogenic medium. Relative quantification to control cells cultured in osteogenic medium. ( a–f ) Average values ± SD are plotted. One-way ANOVA tests, * p < 0.05, ** p < 0.001, *** p < 0.0001, ns—not significant, relative to respective control or between marked groups.

Article Snippet: ASC52telo cells (ASC; ATCC, SCRC-4000) and normal human ASC (ATCC, PCS-500-011) were expanded in the dedicated medium (ATCC, Mesenchymal Stem Cell Basal Medium with Mesenchymal Stem Cell Growth Kit).

Techniques: Activity Assay, Expressing, Derivative Assay, Recombinant, Control, Quantitative Proteomics, Cell Culture

The suggested new signaling pathways induced by Noggin in human ASC osteogenic cultures. We have demonstrated that Noggin can activate FGFR2 receptors and Src kinase associated with the receptor complex. This results in ERK1/2 phosphorylation and, independently of PI3k, Akt kinase phosphorylation. It is known that dexamethasone, a component of osteogenic medium, stimulates RUNX2 and TAZ expressions. We have shown Noggin activation of Akt that leads to blocking the ability of GSK3 to degrade TAZ and suppress RUNX2 activity, thereby stabilizing TAZ and enhancing formation of RUNX2-TAZ complexes. Whereas RUNX2-TAZ complexes can be phosphorylated by Noggin-activated ERK1/2. Such activated RUNX2-TAZ complexes are required for the transcription of osteogenic genes. Besides, we have shown Noggin-related inhibition of SMAD1/5/8 activity, which may be a result of increased SMAD 7 expression due to Akt activity. Figure was created in Affinity Designer software (1.10.6).

Journal: Scientific Reports

Article Title: Noggin promotes osteogenesis in human adipose-derived mesenchymal stem cells via FGFR2/Src/Akt and ERK signaling pathway

doi: 10.1038/s41598-024-56858-w

Figure Lengend Snippet: The suggested new signaling pathways induced by Noggin in human ASC osteogenic cultures. We have demonstrated that Noggin can activate FGFR2 receptors and Src kinase associated with the receptor complex. This results in ERK1/2 phosphorylation and, independently of PI3k, Akt kinase phosphorylation. It is known that dexamethasone, a component of osteogenic medium, stimulates RUNX2 and TAZ expressions. We have shown Noggin activation of Akt that leads to blocking the ability of GSK3 to degrade TAZ and suppress RUNX2 activity, thereby stabilizing TAZ and enhancing formation of RUNX2-TAZ complexes. Whereas RUNX2-TAZ complexes can be phosphorylated by Noggin-activated ERK1/2. Such activated RUNX2-TAZ complexes are required for the transcription of osteogenic genes. Besides, we have shown Noggin-related inhibition of SMAD1/5/8 activity, which may be a result of increased SMAD 7 expression due to Akt activity. Figure was created in Affinity Designer software (1.10.6).

Article Snippet: ASC52telo cells (ASC; ATCC, SCRC-4000) and normal human ASC (ATCC, PCS-500-011) were expanded in the dedicated medium (ATCC, Mesenchymal Stem Cell Basal Medium with Mesenchymal Stem Cell Growth Kit).

Techniques: Protein-Protein interactions, Phospho-proteomics, Activation Assay, Blocking Assay, Activity Assay, Inhibition, Expressing, Software